NASC Stock Detail Page
NASC ID: N16269
Name: pgp4-1
ABRC stock number: CS16269
Description: Confirmed line isolated from original SALK line SALK_063720; homozygous for the insertion. Selected after PCR analysis of the insertion site. PGP4 exhibits apolar plasma membrane localization in the root cap and polar localization in tissues above. Auxin influx transporter shows reversibility in heterologous systems.
Donation Date: 2007-09-19
Donated by: | Purdue University Peter Goldsbrough Purdue University Angus Murphy |
Donor Number: pgp4-1
Part of : Homozygous lines
Stock type: individual line
Material type: seed
Comment: recessive
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000, SALK_063720)
Segregation status: homozygous
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Reduced root gravitropic bending and elongation as well as lateral root formation; reduced root elongation is conditional and dependent on light intensity and sucrose. Reduced basipetal auxin transport in roots (~35%) and a small decrease in shoot-to-root transport consistent with a partial loss of the redirective auxin sink in the root.
References
Terasaka, K. et al. 2005. PGP4, an ATP binding cassette P-glycoprotein, catalyzes auxin transport in Arabidopsis thaliana roots. The Plant cell 17(11):2922-39.PubMed ID: 16243904.